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28. 7. 2024
For the Curious Minds: How EquineTest Whole Genome Sequencing Works?
The horse's DNA or genome is made up of 2.7 billion bases, simplified to the letters A, G, C, T. The combination of these four letters in the DNA strand encodes approximately 20,000 genes that are responsible for the appearance, characteristics and health of the individual.
Until recently, however, no technology was available to read the 2.7 billion letters of DNA quickly and cost-effectively. But the latest sequencing technologies now allow us to do just that.
The very first step, after isolating your horse's DNA, is to break that DNA into small fragments and create what is called a sequencing library. We then read the fragments in this library one by one using a device called a sequencer to identify the order of the bases (A, T, C, G) in the fragment. In a third step, we assemble these short fragments using computer programs to get a complete picture of the genome of the test horse. Finally, we compare the information obtained with a reference genome and, on the basis of this comparison, we can then diagnose genetic diseases, predisposition to diseases or identify genetic variants associated with certain traits.
Thinking of Whole Genome Sequencing, think of assembling a puzzle. The result is a comprehensive genetic map that reveals the secrets hidden within. It is not just science; it is a journey of discovery for every horse enthusiast.
For more details of the procedure please contact us.
